Title

A Novel Immortalized Human Endometrial Stromal Cell Line with Normal Progestational Response

Document Type

Article

Publication Date

2004

MeSH Terms

Endometrium, Stromal cells, Cell Line, Transformed, Telomerase/metabolism, Insulin-Like Growth Factor Binding Protein 1/genetics

Subject: LCSH

Endometrium, Cell lines

Disciplines

Biology | Ecology and Evolutionary Biology

Abstract

Obtaining primary human endometrial stromal cells (HESCs) for in vitro studies is limited by the scarcity of adequate human material and the inability to passage these cells in culture for long periods. Immortalization of these cells would greatly facilitate studies; however, the process of immortalization often results in abnormal karyotypes and aberrant functional characteristics. To meet this need, we have introduced telomerase into cultured HESCs to prevent the normal shortening of telomeres observed in adult somatic cells during mitosis. We have now developed and analyzed a newly immortalized HESC line that contains no clonal chromosomal structural or numerical abnormalities. In addition, when compared with the primary unpassaged parent cells, the new cell line displayed similar biochemical endpoints after treatment with ovarian steroids. Classical decidualization response to estradiol plus medroxyprogesterone acetate were seen in both morphologically, and progestin was seen to induce or regulate the expression of IGF binding protein-1, fibronectin, prolactin, tissue factor, plasminogen activator inhibitor-1, and Fas/Fas ligand. In summary, an immortalized HESC line has been developed that is karyotypically, morphologically, and phenotypically similar to the primary parent cells, and it is a powerful and consistent resource for in vitro work.

Comments

© 2004 The Endocrine Society. For personal use only. No other uses without permission. All rights reserved.

DOI

10.1210/en.2003-1606#sthash.OKy5XNYb.dpuf

Publisher Citation

Krikun, G., Mor, G., Alvero, A., Guller, S., Schatz, F., Sapi, E., Rahman, M., Caze, R., Rebeca Caze, Qumsiyeh, M., & Lockwood, C. J. (2004). A novel immortalized human endometrial stromal cell line with normal progestational response. Endocrinology, 145(5), 2291-2296.