Document Type

Article

Publication Date

2-28-2014

Subject: LCSH

Nitric oxide, Blood platelets, Extracorporeal circulation

Disciplines

Biomedical Engineering and Bioengineering | Mechanical Engineering

Abstract

Nitric oxide (NO) is an endogenous vasodilator as well as natural inhibitor of platelet adhesion/ activation. Nitric oxide releasing (NOrel) materials can be prepared by doping an NO donor species, such as diazeniumdiolated dibutylhexanediamine (DBHD/N2O2), within a polymer coating. The inherent hemocompatibility properties of the base polymer can also influence the efficiency of such NO release coatings. In this study, four biomedical grade polymers were evaluated in a 4 h rabbit model of thrombogenicity for their effects on extracorporeal circuit thrombus formation and circulating platelet count. At the end of 4 h, Elast-Eon E2As was found to preserve 58% of baseline platelets versus 48, 40, and 47% for PVC/DOS, Tecophilic SP-60D-60, and Tecoflex SG80A, respectively. Elast-Eon also had significantly lower clot area of 5.2 cm2 compared to 6.7, 6.1, and 6.9 cm2 for PVC/DOS, SP-60D-60, and SG80A, respectively. Based on the results obtained for the base polymer comparison study, DBHD/N2O2-doped E2As was evaluated in short-term (4 h) rabbit studies to observe the NO effects on prevention of clotting and preservation of platelet function. Platelet preservation for this optimal NO release formulation was 97% of baseline after 4 h, and clot area was 0.9 cm2 compared to 5.2 cm2 for controls, demonstrating that combining E2As with NO release provides a truly advanced hemocompatible polymer coating for extracorporeal circuits and potentially other blood contacting applications.

Comments

This is the authors' accepted manuscript. Published in final edited form as: J Mater Chem B Mater Biol Med. 2014 February 28; 2(8): 1059–1067. doi:10.1039/C3TB21771J.

DOI

10.1039/C3TB21771J

Publisher Citation

Hitesh Handa, Terry C. Major, Elizabeth J. Brisbois, Kagya A. Amoako, Mark E. Meyerhoff, and Robert H. Bartlett. J Mater Chem B Mater Biol Med. 2014 February 28; 2(8): 1059–1067. doi:10.1039/C3TB21771J.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.