Document Type

Article

Publication Date

11-2014

Subject: LCSH

Biomimetics--Biotechnology

Disciplines

Biomedical Engineering and Bioengineering | Mechanical Engineering

Abstract

This study examines platelet adhesion on surfaces that combine coatings to limit protein adsorption along with “anti-platelet” nitric oxide (NO) release. Uncoated and poly-2-methoxyethylacrylate (PMEA) coated, gas permeable polypropylene (PP) membranes were placed in a bioreactor to separate plasma and gas flows. Nitrogen with 100/500/1000 ppm of NO was supplied to the gas side as a proof of concept. On the plasma side, platelet rich plasma (PRP, 1 × 108 cell/mL) was recirculated at low (60)/high (300) flows (mL/min). After 8 hours, adsorbed platelets on PP was quantified via a lactate dehydrogenase assay. Compared to plain PP, the PMEA coating alone reduced adsorption by 17.4 ± 9.2% and 29.6 ± 16.6% at low and high flow (p < 0.05), respectively. NO was more effective at low plasma flow. At 100 and 500 ppm of NO, adsorption fell by 37.9 ± 6.1% and 100 ± 4.7%, (p < 0.001), on plain PP. At high flow with 100, 500, and 1000 ppm of NO, adsorption reduced by 17.9 ± 17.8%, 46.4 ± 23.2%, and 100 ± 4.8%, (p < 0.001), respectively. On PMEA-coated PP with only 100 ppm, adsorption fell by 69.7 ± 6.8 and 65.6% ± 16.9%, (p < 0.001), at low and high flows respectively. Therefore, the combination of an anti-adsorptive coating with NO has great potential to reduce platelet adhesion and coagulation at biomaterial surfaces.

Comments

This is the accepted version of the following article: Gupta, S., Amoako, K. A., Suhaib, A., Cook, K. E. (2014). Multi-Modal, Surface-Focused Anticoagulation Using Poly-2-methoxyethylacrylate Polymer Grafts and Surface Nitric Oxide Release. Adv. Mater. Interfaces, 1: 1400012. which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/admi.201400012/abstract

DOI

10.1002/admi.201400012

Publisher Citation

Surbhi Gupta, Kagya A. Amoako, Ahmed Suhaib, Keith E. Cook. Multi-modal, surface focused anticoagulation using poly-2-methoxyethylacrylate polymer grafts and surface nitric oxide release. Advanced Material Interfaces. Volume 1, Issue 8, November, 2014, 1:1400012. doi: 10.1002/admi.201400012.

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