Author URLs
Professor Amoako's faculty profile
Biomaterials & Medical Device Innovation Laboratory (BMD iLab)
Document Type
Article
Publication Date
12-2013
MeSH Terms
Thrombosis, Nitric Oxide, Extracorporeal Circulation, Silicones, Biocompatibility Testing
Subject: LCSH
Polymers in medicine, Oxygenators, Nitric oxide
Disciplines
Biomedical Engineering and Bioengineering | Mechanical Engineering
Abstract
Hollow fiber artificial lungs are increasingly being used for long-term applications. However, clot formation limits their use to 1–2 weeks. This study investigated the effect of nitric oxide generating (NOgen) hollow fibers on artificial lung thrombogenicity. Silicone hollow fibers were fabricated to incorporate 50 nm copper particles as a catalyst for NO generation from the blood. Fibers with and without (control) these particles were incorporated into artificial lungs with a 0.1 m2 surface area and inserted in circuits coated tip-to-tip with the NOgen material. Circuits (N = 5/each) were attached to rabbits in a pumpless, arterio-venous configuration and run for 4 h at an activated clotting time of 350–400 s. Three control circuits clotted completely, while none of the NOgen circuits failed. Accordingly, blood flows were significantly higher in the NOgen group (95.9 ± 11.7, p < 0.01) compared to the controls (35.2 ± 19.7; mL/min), and resistance was significantly higher in the control group after 4 h (15.38 ± 9.65, p < 0.001) than in NOgen (0.09 ± 0.03; mmHg/mL/min). On the other hand, platelet counts and plasma fibrinogen concentration expressed as percent of baseline in control group (63.7 ± 5.7%, 77.2 ± 5.6%; p < 0.05) were greater than those in the NOgen group (60.4 ± 5.1%, 63.2 ± 3.7%). Plasma copper levels in the NOgen group were 2.8 times baseline at 4 h (132.8 ± 4.5 μg/dL) and unchanged in the controls. This study demonstrates that NO generating gas exchange fibers could be a potentially effective way to control coagulation inside artificial lungs.
DOI
10.1002/jbm.a.34655
Repository Citation
Amoako, Kagya; Montoya, Patrick; Major, Terry C.; Suhaib, Ahmed B.; Handa, Hitesh; Brant, David O.; Meyerhoff, Mark E.; Bartlett, Robert H.; and Cook, Keith E., "Fabrication and In vivo Thrombogenecity Testing of Nitric Oxide Generating Artificial Lungs" (2013). Mechanical and Industrial Engineering Faculty Publications. 6.
https://digitalcommons.newhaven.edu/mechanicalengineering-facpubs/6
Publisher Citation
KA Amoako, Montoya JP, Major TC, Meyerhof ME, Bartlett RH, Cook KE. Fabrication and In vivo Thrombogenecity Testing of Nitric Oxide Generating Artificial Lungs. J Biomed Mater Res A. 2013, 101(12): 3511-3519
Comments
This is the pre-peer reviewed version of the following article: KA Amoako, Montoya JP, Major TC, Meyerhof ME, Bartlett RH, Cook KE. Fabrication and In vivo Thrombogenecity Testing of Nitric Oxide Generating Artificial Lungs. J Biomed Mater Res A. 2013, 101(12): 3511-3519, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.34655/abstract . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.