Lyme disease, Borrelia burgdorferi, Microscopy, Atomic Force
Borrelia burgdorferi, Atomic force microscopy
Biology | Ecology and Evolutionary Biology
Bacterial biofilms are microbial communities held together by an extracellular polymeric substance matrix predominantly composed of polysaccharides, proteins and nucleic acids. We had previously shown that Borrelia burgdorferi sensu stricto, the causative organism of Lyme disease in the United States is capable of forming biofilms in vitro. Here, we investigated biofilm formation by B. afzelii and B. garinii, which cause Lyme disease in Europe. Using various histochemistry and microscopy techniques, we show that B. afzelii and B. garinii form biofilms, which resemble biofilms formed by B. burgdorferisensu stricto. High-resolution atomic force microscopy revealed similarities in the ultrastructural organization of the biofilms form by three Borrelia species. Histochemical experiments revealed a heterogeneous organization of exopolysaccharides among the three Borrelia species. These results suggest that biofilm formation might be a common trait of Borrelia genera physiology.
Timmaraju, Venkata Arun; Theophilus, Priyanka A.S.; Balasubramanian, Kunthavai; Shakih, Shafiq; Luecke, David F.; and Sapi, Eva, "Biofilm Formation by Borrelia Burgdorferi Sensu Lato" (2015). Biology and Environmental Science Faculty Publications. 36.
Biofilm formation by Borrelia burgdorferi sensu lato. Venkata Arun Timmaraju, Priyanka A. S. Theophilus, Kunthavai Balasubramanian, Shafiq Shakih, David F. Luecke, Eva Sapi. FEMS Microbiology Letters Volume 362, Issue 15. DOI: http://dx.doi.org/10.1093/femsle/fnv120 fnv120 First published online: 24 July 2015