Author URLs
Document Type
Article
Publication Date
10-9-2018
MeSH Terms
Blood Coagulation, Fibrinogen
Subject: LCSH
Polyzwitterions, Fibrinogen, Blood--Coagulation
Disciplines
Industrial Engineering | Mechanical Engineering
Abstract
The effect of surface coatings on the performance of antifouling activity under flow can be influenced by the flow/coating interactions. This study evaluates the effect of surface coatings on antifouling activity under different flows for the analyses of coating stability. This was done by exposing DOPA-PCB-300/dopamine coated polydimethylsiloxane (PDMS) to physiological shear stresses using a recirculation system which consisted of dual chamber acrylic flow cells, tygon tubing, flow probe and meter, and perfusion pumps. The effect of shear stress induced by phosphate buffered saline flow on coating stability was characterized with differences in fibrinogen adsorption between control (coated PDMS not loaded with shear stress) and coated samples loaded with various shear stresses. Fibrinogen adsorption data showed that relative adsorption on coated PDMS that were not exposed to shear (5.73% ± 1.97%) was significantly lower than uncoated PDMS (100%, p < 0.001). Furthermore, this fouling level, although lower, was not significantly different from coated PDMS membranes that were exposed to
1 dyn/cm2 (9.55% ± 0.09%, p = 0.23), 6 dyn/cm2 (15.92% ± 10.88%, p = 0.14), and 10 dyn/cm2 (21.62% ± 13.68%, p = 0.08). Our results show that
DOPA-PCB-300/dopamine coatings are stable, with minimal erosion, under shear stresses tested. The techniques from this fundamental study may be used to determine the limits of stability of coatings in long-term experiments.
DOI
10.1021/acs.langmuir.8b03078
Repository Citation
Belanger, Andrew; Decarmine, Andre; Jiang, Shaoyi; Cook, Keith; and Amoako, Kagya, "Evaluating the Effect of Shear Stress on Graft-To Zwitterionic Polycarboxybetaine Coating Stability Using a Flow Cell" (2018). Mechanical and Industrial Engineering Faculty Publications. 40.
https://digitalcommons.newhaven.edu/mechanicalengineering-facpubs/40
Publisher Citation
Belanger, A., Decarmine, A., Jiang, S., Cook, K., & Amoako, K. A. (2018). Evaluating the Effect of Shear Stress on Graft-To Zwitterionic Polycarboxybetaine Coating Stability Using a Flow Cell. Langmuir. doi:10.1021/acs.langmuir.8b03078
Comments
This is the authors' submitted, preprint version of the article published in Langmuir by the American Chemical Society. The version of record can be found at
http://dx.doi.org/10.1021/acs.langmuir.8b03078. This article is part of the Zwitterionic Interfaces: Concepts and Emerging Applications special issue.