Faculty Mentor

Ali Senejani


Biology and Environmental Sciences



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Chlorine Dioxide (ClO2) is a synthetic, green-yellowish gas with a chlorine-like, irritating odor that is used for the treatment of drinking water and food preservation. The aim of this study was to evaluate the genotoxic potential of the chlorine dioxide in mammalian cells and to determine what dose of ClO2 is tolerable by normal cells and cells with aberrant DNA repair genes. Human and mouse embryonic cells were treated with various ClO2 dilutions ranging from 2.5 mM to 25 mM. The cell viability and metabolic activity was determined via MTT, a colorimetric assay. Human HEK293 cells exposed to 5mM of chlorine dioxide exhibit rapid cell death. Results from the alkaline comet assay indicate cells treated with a 50 mM ClO2 carry high levels of DNA breaks. Furthermore, mouse embryonic fibroblast cells lacking a key DNA repair gene, DNA polymerase beta, show an increased level of sensitivity to ClO2 indicating its use as a potential chemotherapy approach for cancer cells harboring defective DNA repair genes.

Publication Date



University of New Haven


West Haven


cancer, chemotherapy, Chlorine Dioxide, HEK293, MEF, comet assays, DNA polymerase beta

Subject: LCSH

Cancer, Chemotherapy, Chlorine dioxide

Subject: MeSH

Neoplasms/drug therapy, HEK293 Cells


Biology | Neoplasms


A special note of gratitude to the SURF support team at the University of New Haven, particularly Mrs. Carol Withers. I also want to thank Dr. Ali Senejani for helping me design the whole model for this research and guiding me with useful strategies. I find myself absolutely grateful for all his time, patience and 100% availability. Lastly, I would like to acknowledge graduate students Beatrice Doubert, Joseph Magrino and George Tsai, who helped me unconditionally with my many inquiries during the research.

Evaluating Human Cell Survival and DNA Damage After Exposure to Various Amounts of Chlorine Dioxide and Exploring Its Use as a Potential Cancer Chemotherapy Agent