Author URLs
Document Type
Article
Publication Date
2016
MeSH Terms
Colorectal Neoplasms, MicroRNAs
Subject: LCSH
Colon (Anatomy)--Cancer, MicroRNA
Disciplines
Forensic Science and Technology | Gastroenterology | Genetics and Genomics | Oncology
Abstract
BACKGROUND: Cells release extracellular membrane vesicles including microvesicles known as exosomes. Exosomes contain microRNAs (miRNAs) however the full range within colorectal cancer cell secreted exosomes is unknown. OBJECTIVE: To identify the full range of exosome encapsulated miRNAs secreted from 2 colorectal cancer cell lines and to investigate engineering of exosomes over-expressing miRNAs. METHODS: Exosomes were isolated from HCT-116 and HT-29 cell lines. RNA was extracted from exosomes and microRNA array performed. Cells were engineered to express miR-379 (HCT-116-379) or a non-targeting control (HCT-116-NTC) and functional effects were determined. Exosomes secreted by engineered cells were transferred to recipient cells and the impact examined. RESULTS: Microvesicles 40-100 nm in size secreted by cell lines were visualised and confirmed to express exosomal protein CD63. HT-29 exosomes contained 409 miRNAs, HCT-116 exosomes contained 393, and 338 were common to exosomes from both cell lines. Selected targets were validated. HCT-116-379 cells showed decreased proliferation (12-15% decrease, p < 0.001) and decreased migration (32-86% decrease, p < 0.001) compared to controls. HCT-116-379 exosomes were enriched for miR-379. Confocal microscopy visualised transfer of HCT-116-379 exosomes to recipient cells. CONCLUSIONS: Colorectal cancer cells secrete a large number of miRNAs within exosomes. miR-379 decreases cell proliferation and migration, and miR-379 enriched exosomes can be engineered.
DOI
10.3233/CBM-160659
Repository Citation
Clancy, Cillian; Khan, Sonja; Glynn, Claire L.; Holian, Emma; Dockery, Peter; Lalor, Pierce; Brown, James A.L.; Joyce, Myles; Kerin, Michael J.; and Dwyer, Roisin M., "Screening of Exosomal MicroRNAs From Colorectal Cancer Cells" (2016). Forensic Science Publications. 24.
https://digitalcommons.newhaven.edu/forensicscience-facpubs/24
Publisher Citation
Clancy, Cillian, Sonja Khan, Claire L. Glynn, Emma Holian, Peter Dockery, Pierce Lalor, James AL Brown, Myles R. Joyce, Michael J. Kerin, and Roisin M. Dwyer. "Screening of exosomal microRNAs from colorectal cancer cells." Cancer Biomarkers Preprint (2016): 1-9.
Included in
Forensic Science and Technology Commons, Gastroenterology Commons, Genetics and Genomics Commons, Oncology Commons
Comments
This is the authors' accepted version of the article published in Cancer Biomarkers. The final publication is available at IOS Press through
http://dx.doi.org/10.3233/CBM-160659 .