Quality Control and Duplication for Concordance in Forensic DNA Samples: Implications for Interpretation in Mixtures

Document Type

Article

Publication Date

6-2015

MeSH Terms

DNA, Forensic Sciences

Subject: LCSH

DNA, Forensic Sciences

Disciplines

Forensic Science and Technology

Abstract

In DNA human identification, concordance between duplicate samples is required for quality assurance of results. When variation is noted, the underlying science points to factors such as sub-optimal DNA template concentration, potential contamination events due to primary or secondary transfer, and DNA degradation or breakage. All of these factors relate back to PCR amplification efficiency which also influences the error rates for the reliable detection of alleles. For low level DNA mixtures or interpretation of major donor alleles in a mixture, PCR amplification kinetics and detection of data close to the baseline of the instrumentation is critical for any attempt to establish the true number of contributors to a sample. With software, quantitative analysis of run to run variation can be established by evaluating peak height differences and is useful as an aid in DNA mixture interpretation.

Comments

This article appeared in the June 2015 issue of International Research Journal of Computer Science (IRJCS).

The full-text of this article is available at www.irjcs.com.

Publisher Citation

Coyle, H. (2015). Quality Control and Duplication for Concordance in Forensic DNA Samples: Implications for Interpretation in Mixtures. IRJCS:: International Research Journal of Computer Science, Volume II, 16-18.

Link to Full Text

Share

COinS