Author URLs
Professor Amoako's Faculty Profile
Biomaterials & Medical Device Innovation Laboratory (BMD iLab)
Document Type
Article
Publication Date
1-19-2016
MeSH Terms
Biotechnology, Dimethylpolysiloxanes, Nitric Oxide, polycarboxybetaine methacrylate
Subject: LCSH
Biotechnology, Polydimethylsiloxane, Nitric oxide
Disciplines
Biomedical Engineering and Bioengineering | Mechanical Engineering
Abstract
The functions of anti-fouling, zwitterionic polycarboxybetaine (pCB) and anti-platelet nitric oxide (NO) release replicate key anticoagulant properties of the endothelium. The two approaches, only tested separately thus far, were paired on gas permeable polydimethylsiloxane (PDMS) membranes and evaluated for anti-coagulation. Uncoated PDMS (control) and PDMS coated with pCB were screened for fibrinogen (Fg) fouling followed by platelet adsorption testing to evaluate the effects of coating and/or NO using bioreactors. Bare or coated PDMS membranes separated sheep plasma (108 platelets/ml) and gas flow chambers within the bioreactors. Either 100 or 0 ppm of NO/N2 flowed through the gas chamber for NO release at the plasma/biomaterial interface. Surface-adsorbed platelets were quantified using a lactate dehydrogenase assay after 8 hrs of plasma recirculation. Fg fouling and platelet adsorption on pCB-coated PDMS were 10.40 ± 3.0% of control (p
DOI
10.1002/admi.201500646
Repository Citation
Amoako, Kagya; Sundaram, Harihara S.; Suhaib, Ahmed; Jiang, Shaoyi; and Cook, Keith E., "Multimodal, Biomaterial-Focused Anticoagulation via Superlow Fouling Zwitterionic Functional Groups Coupled with Anti-Platelet Nitric Oxide Release" (2016). Mechanical and Industrial Engineering Faculty Publications. 10.
https://digitalcommons.newhaven.edu/mechanicalengineering-facpubs/10
Publisher Citation
Amoako, K. A., Sundaram, H. S., Suhaib, A., Jiang, S. and Cook, K. E. (2016), Multimodal, Biomaterial-Focused Anticoagulation via Superlow Fouling Zwitterionic Functional Groups Coupled with Anti-Platelet Nitric Oxide Release. Advanced Materials Interfaces. Article first published online: 19 JAN 2016 doi: 10.1002/admi.201500646
Comments
This is the peer reviewed version of the following article: Amoako, K. A., Sundaram, H. S., Suhaib, A., Jiang, S. and Cook, K. E. (2016), Multimodal, Biomaterial-Focused Anticoagulation via Superlow Fouling Zwitterionic Functional Groups Coupled with Anti-Platelet Nitric Oxide Release. Advanced Materials Interfaces, which has been published in final form at http://dx.doi.org/10.1002/admi.201500646. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.