Document Type

Article

Publication Date

2011

MeSH Terms

Nitric oxide, Polymers, Blood Coagulation

Subject: LCSH

Polymers in medicine, Nitric oxide

Disciplines

Biomedical Engineering and Bioengineering | Mechanical Engineering

Abstract

Coagulation upon blood-contacting biomaterials remains a problem for short and long-term clinical applications. This study examined the ability of copper(II)-doped silicone surfaces to generate nitric oxide (NO) and locally inhibit coagulation. Silicone was doped with 3-micron copper (Cu(0)) particles yielding 3 to 10 weight percent (wt%) Cu in 70-μm thick Cu/Silicone polymeric matrix composites (Cu/Si PMCs). At 3, 5, 8 and 10 wt% Cu doping, the surface expression of Cu was 12.1 ± 2.8%, 19.7 ± 5.4%, 29.0 ± 3.8%, and 33.8 ± 6.5% respectively. After oxidizing Cu(0) to Cu(II) by spontaneous corrosion, NO flux, JNO (mol*cm−2*min−1), as measured by chemiluminescence, increased with surface Cu expression according to the relationship JNO =(1.63 %SACu −0.81) ×10−11, R2 = 0.98 where %SACu is the percentage of surface occupied by Cu. NO flux at 10 wt% Cu was 5.35± 0.74 ×10−10 mol*cm−2*min−1. The clotting time of sheep blood exposed to these surfaces was 80 ± 13s with pure silicone and 339 ± 44s when 10 wt% Cu(II) was added. SEMs of coatings showed clots occurred away from exposed Cu-dendrites. In conclusion, Cu/Si PMCs inhibit coagulation in a dose-dependent fashion related to the extent of copper exposure on the coated surface.

Comments

This is a non-final version of an article published in final form in KA Amoako, Cook KE. Nitric oxide-generating silicone as a blood-contacting biomaterial. ASAIO Journal 2011; 57(6):539–544.

This is the authors' manuscript as accepted for publication.

The version of record is available at http://dx.doi.org/10.1097/MAT.0b013e31823b9692

DOI

10.1097/MAT.0b013e31823b9692

Publisher Citation

KA Amoako, Cook KE. Nitric oxide-generating silicone as a blood-contacting biomaterial. ASAIO Journal 2011; 57(6):539–544

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