Date of Submission
12-2025
Document Type
Thesis
Degree Name
Master of Science in Cellular and Molecular Biology
Department
Biology and Environmental Sciences
Advisor
Nikolas M. Stasulli, Ph.D.
Committee Member
Anna Kloc, Ph.D.
Committee Member
Martha Anne Taddeo Ph.D.
Keywords
Bacterial Interactions, Luteibacter Anthropi, Streptomyces Viridochromogenes, Sporulation, Sporulation-Inhibiting Metabolite, Lanthipeptide-Class-ii Genome Cluster
MeSH
Microbial Interactions, Gammaproteobacteria, Streptomyces, Bacteriocins
LCSH
Bacteria, Streptomyces, Microbial inactivation, Microbial metabolites, Microbial genetics
Abstract
Interactions between bacterial species have been an up and coming area of scientific research, as the transcriptional changes leading to phenotypic changes in many of these interactions have yet to be fully characterized. One of these interactions was discovered between an environmental isolate of Luteibacter anthropi and a type strain of Streptomyces viridochromogenes. Many Streptomyces species undergo a sporulation process during reproductive growth, but when grown together with L. anthropi, S. viridochromogenes' reproductive sporulation is inhibited. The chemical components responsible for said interaction have not yet been determined. This experimental design aimed to characterize the chemical components responsible for the inhibition of sporulation during the interactions between these bacteria using analytical techniques, including infrared spectroscopy and ultraviolet spectroscopy, to further characterize how this interaction is mediated. The analytical results were cross referenced with the genomic sequence of L. anthropi to identify potential gene clusters that may produce the specialized metabolite responsible for sporulation inhibition. Results suggest that the responsible compound is a small secondary metabolite produced by a lanthipeptide-class-ii genome cluster that contains a high concentration of alcohol functional groups and polar amino acids, such as serine and threonine.
Recommended Citation
Schwab, Aiden Eli, "Isolation and Structural Prediction of a Luteibacter Anthropi Specialized Metabolite produced in Competition with Streptomyces Viridochromogenes" (2025). Master's Theses. 291.
https://digitalcommons.newhaven.edu/masterstheses/291